Hdl cholesterol test procedure pdf

Seven direct methods for measuring hdl and ldl cholesterol. High density lipoprotein structurefunction and role in. Total cholesterol determination and hdlcholesterol. Hdl cholesterol highdensity lipoprotein this type of cholesterol can bind the fat in the body and carry it to the liver for flushing out. The wako hdlcholesterol e is an in vitro assay for the quantitative. An hdl cholesterol test or a high density lipoprotein cholesterol test may be recommended to determine your risk of developing heart disease. The lipid panel is used as part of a cardiac risk assessment to help determine your risk of heart disease and to help make decisions about what treatment may be best if you have borderline risk, intermediate. Control sera are recommended to monitor the performance of manual and. The test combines two steps, the first step eliminating chylomicrons, vldl and ldl by specific enzymatic degradation. The hdlcholesterol test is a two reagent homogenous system for the selective. Lipid profile total cholesterol, hdlcholesterol, triglyceride, calculated ldl these assays are run at the clinical chemistry laboratory at fletcher allen health care, an affiliate of the university of vermont. Nonhdl cholesterol is the total of vldl and ldl cholesterol, both of which contain atherogenic apolipoprotein b apo b particles. Add 50 \\mu\l of supernate from precipitation procedure to hdl control and hdl serum sample tubes, add 50.

We previously developed an assay to directly measure small dense sd lowdensity lipoprotein cholesterol ldlc levels, which is not widely used in general clinical practice. High density lipoprotein hdl is the fraction of plasma lipoprotein with a hydrated density of 1. Fujifilm wako diagnosticshdl cholesterol manual reagent. The test has been developed to determine hdlc concentrations within a measuring range from. Samples should be diluted with physiological saline, the assay repeated and the result multiplied by the dilution factor whenever. Manual testing procedure for cholesterol determination. Because it approximates the amount of apo b particles, nonhdl. All stanbio chemistry reagents ekf diagnostics usa. Generally fasting for 9 to 12 hours is required before testing but drinking water is allowed. Total cholesterol, hdlcholesterol, triglycerides cdc. Coronary risk increases markedly as the hdl concentration. A complete cholesterol test also called a lipid panel or lipid profile is a blood test that can measure the amount of cholesterol and triglycerides in your blood.

Hdlcholesterol in serum on the hitachi 717 nhanes 20052006 1. Following is the specimen collection process for hdl cholesterol blood test. It appears that hdl particles scour the walls of blood vessels, cleaning. Including direct clearance and phosphotungstic acid. Alere cholestech ldx system waived laboratory procedure. Laboratory procedure manual centers for disease control.

Also known as the bad cholesterol, ldl is the main source. The current national guidelines on the use of ldl cholesterol ldlc 10 and hdlc for cardiovascular risk assessment 1, 2 were largely based on early epidemiologic studies that established the link. A lipid profile including total cholesterol tc, highdensity lipoprotein cholesterol hdlc, ldlc, and triglycerides tgs should be assessed in all patients with suspected or known stable cad. Hdl cholesterol direct enzymatic colorimetric quantitative. The focus in this chapter is on the antiatherogenic lipoprotein, high density lipoprotein hdl, which mediates the efflux of cellular cholesterol. A cholhdl ratio and estimated values for ldl cholesterol. In nhanes 20052006, hdlcholesterol is measured directly in serum without. Follow steps 1 and 2 of total cholesterol procedure. High levels of hdl cholesterol, often called good cholesterol, are associated with a reduced risk of coronary artery disease cad. If you have a borderline high total cholesterol along with hdl 60, no treatment is required because a high hdl level is considered protective. The hdlcholesterol is acted upon by pegcholesterol oxidase, and the hydrogen peroxide produced from this reaction combines with 4aminoantipyrine and hsda under the action of peroxidase to form a. There are several methods available for measurement of hdl cholesterol. This is referred to as good cholesterol because it helps remove ldl cholesterol from your blood.

Direct hdlcholesterol liquicolor materials required but. The doctor may or may not advise against intake of chronic medication before the test. The highdensity lipoprotein test or the hdl test measures the amount of good cholesterol in the body of the patient. For hdl cholesterol, or good cholesterol, higher levels are better. Testing with various concentrations of ds solution 630 mgml in the course of the.

High density lipoprotein hdl cholesterol low serum concentrations of hdlcholesterol are associated with increased risk for chd. Cholesterol test reasons, who needs it, testing procedure. Hdl highdensity lipoprotein cholesterol is actually the good cholesterol. Cholesterol, as we know, is found in all the cells of the body. This procedure is intended to provide a ready outline reference for performance o these f the assay. The truechemie direct hdl cholesterol test kit for the in vitro quantitative. Cell biolabs hdlcholesterol assay kit measures the hdl cholesterol levels in serum or plasma samples. Hdl particles carry cholesterol from the cells back to the liver. It is calculated by adding a participants hdl and ldl level, plus 20% of their triglyceride level. How is the specimen collected for hdl cholesterol blood test. Nonhdl cholesterol this level is found by subtracting your. A cholesterol test can give your health care provider important information about the cholesterol levels in your blood. New device product code regulation name classification regulation section. Hdl is known as good cholesterol because high levels are thought to lower the risk of heart disease.

See how your cholesterol numbers compare to the tables below. How to prepare do not drink alcohol in the 24 hours prior to the test. A hdl cholesterol test may be used with other cholesterol levels. Hdlcholesterol expected values in serum hdlcholesterol. This is a good cholesterol and a high level of this can. Hdlcholesterol values constitute an independent risk factor. Highdensity lipoprotein hdl cholesterol is known as the. Hdl participates in the reverse cholesterol transport. Results of studies conducted7 show that the following substances interfere with this hdlcholesterol procedure. Your cholesterol levels are an important measure of heart health. It is also used to diagnose cholesterol level anomalies. An hdl level of 60 mgdl milligrams of cholesterol per deciliter of blood or above is associated with a lower risk.